The culture of excised organs in a suitable aseptic medium. Roots, leaves, embryos, meristems, and many other plant structures have been successfully maintained in culture. By observing growth in the presence and absence of various nutrients, valuable information about the physiology of the organ can be obtained. For example, chemical factors affecting sex expression have been studied using cultures of isolated flowers, and root nodule formation has been investigated in cultures of legume roots. See also meristem culture , ovule culture , embryo culture. |
